Researchers reveal how intestinal bacteria affects growth of fungi

Story by  IANS | Posted by  shaista fatima | Date 15-05-2023
A representational image
A representational image



The bacteria in the intestine provide information on the amounts of fungi from the possibly pathogenic Candida genus. Surprisingly, among them are lactic acid bacteria, which are known for their ability to guard against fungal diseases.

Researchers from the Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and its Danish and Hungarian collaborators add another piece to the puzzle of comprehending the human gut microbiome.

The human gut microbiome is an exceedingly complicated community in which many microbes regulate one another. Individual species can spread and cause infection if an imbalance exists as a result of antibiotics or other environmental effects. Candida fungi, for example, are found in the intestines of many healthy persons. They are normally harmless, but they have the potential to induce serious systemic infections.

Studying these interactions in the intestine is difficult. The several hundred species of bacteria and fungi can only be partially cultivated in the laboratory, and many are not even known. Researchers at the Leibniz-HKI are therefore trying to shed more light on the intestine using metagenome studies. For the study now published in Nature Communications, the researchers examined stool samples from 75 cancer patients and found that certain bacterial species always appear in greater numbers when the amount of fungi from the Candida genus is also high.

"With these data, we developed a computer model that was able to predict the amount of Candida in another group of patients with an accuracy of about 80 per cent based on bacterial species and amounts alone," explained Bastian Seelbinder, lead author of the study. These bacteria included mainly oxygen-tolerant species. Seelbinder conducts research in Gianni Panagiotou's Microbiome Dynamics department at Leibniz-HKI, which focuses intensively on the gut microbiome.

What surprised the researchers was not only how successful the prediction of the amount of fungi based on the bacterial species present was, but also which bacteria correlated with high amounts of fungi. "We found an increased number of bacterial species that produce lactic acid, including Lactobacillus species," Seelbinder explains. It's a finding he had not expected. "I could hardly believe it at first, so I checked several times, always with the same result."

The reason for his surprise: Several studies have attested to the protective effect of lactic acid bacteria against fungal infections. One of them was published last year by Panagiotou's group, also in the journal Nature Communications. "The result shows once again how complex the human gut microbiome is and how difficult it is to decipher the interactions of different microorganisms," Panagiotou said.

The researchers' hunch: Lactic acid bacteria, particularly of the genus Lactobacillus, favour Candida proliferation but at the same time make the fungus less virulent. This could be due to the fact that Candida species can switch their metabolism to be able to use the lactate produced by lactic acid bacteria. This gives them a competitive advantage over other fungi such as Saccharomyces cerevisiae, as the researchers discovered in additional experiments.

However, the metabolic switch also apparently causes Candida to remain in its usually harmless spherical yeast form instead of forming fungal hyphae that could invade the intestinal mucosa. "There is also a suggestion that certain groups of Lactobacillus species might have different effects," Seelbinder said. To investigate this, the next step will be to perform more detailed genomic analyses of the bacteria.

"For the current study, we examined stool samples from cancer patients who are particularly at risk for fungal infections," Panagiotou explains. For further studies, samples from healthy subjects could be included to develop long-term strategies for at-risk patients based on their microbiome.